How do I order BD? AbSeq Custom Antibodies?
What custom antibody-oligonucleotide conjugation services are currently offered by BD?
BD Biosciences offers the small-scale custom conjugation of an AbSeq qualified oligonucleotide barcode to either antibodies provided by customers or BD antibody clones not currently commercialized in the AbSeq portfolio.
How much does the service cost for customer provided antibody AbSeq conjugation?
The cost of service for conjugating a customer provided antibody with an oligo is $1,350 US per material.
Is there a minimum amount of antibody or specific material requirements that must be met for the conjugation service?
We recommend that customers provide at least 0.4 mg of antibody at a concentration of greater than or equal to 2.0 mg/mL. This is the minimum required amount for our validated BD? AbSeq Custom Conjugation production process. Please note the material provided must be free of BSA and any other carrier proteins.
Is there a minimum or maximum number of custom reagents required per order?
There is no minimum or limit to the number of conjugates requested per order.
What is the expected yield for the customer-provided antibody BD? AbSeq Custom Conjugation?
We cannot guarantee the yield; however, historically it is between 40–50% of the starting amount.
Can I order a BD antibody for AbSeq Custom Conjugation that is not currently included in the AbSeq product line?
Yes, requests will be evaluated on a case-by-case basis assessing technical compatibility. BD? AbSeq Custom Conjugation materials are offered at an optimal concentration, with 25 x 2 µL tests per vial. The optimal concentration is derived from titration analysis via flow cytometry using a fluorochrome labeled format of the antibody.
What is the cost for a BD? AbSeq Custom Conjugation?
The cost of a BD? AbSeq Custom Conjugate of an existing BD clone is $585 US per 25 test size.
Can I order a BD intracellular antibody or provide an intracellular antibody for BD? AbSeq Custom Conjugation?
We have not fully tested and validated the performance of intracellular markers in the AbSeq workflow, therefore do not currently support AbSeq Antibodies against intracellular targets. However, if a customer still wishes to procure an intracellular antibody in AbSeq format, we are happy to support this request via our Customs Conjugation team on a case-by-case basis. Fulfillment of such requests requires acknowledgement of above risks from the requesting customer. We currently do not offer any technical support for Intracellular AbSeq workflow.
What is the lead time for BD? AbSeq Custom Conjugations?
Lead times are typically 4 weeks. We will communicate approximate lead times when the quote is made, as well as provide follow up communication regarding expected dates of delivery.
What formulation will the BD? AbSeq Custom Antibody Conjugates be delivered in?
BD will provide the entire yield of the custom conjugation for customer provided material at a standard concentration of 0.5 mg/mL. If the material is from a BD clone it will be provided at a 25 test size. The suggested working concentration is 2.0 µl/test reaction. An alternative formulation can be provided upon request from the customer.
Can I specify the oligo sequence conjugated to an antibody in a customs order?
BD? AbSeq oligonucleotide barcodes are quality controlled and validated to ensure optimal performance and compatibility. At this time, we cannot accommodate specific AbSeq barcode pairing. For customer-provided materials, assigned oligonucleotide barcodes are unique to each customer incorporated into BD bioinformatics tools for seamless analysis.
Can I design the sequence for the oligonucleotide label tag for BD to use?
BD? AbSeq oligonucleotide barcodes are quality controlled and validated to ensure optimal performance and compatibility. All AbSeq barcodes are incorporated into BD bioinformatics tools for seamless analysis. Unfortunately, we do not currently offer custom conjugation service for alternative oligonucleotide sequences.
How can I get the oligo sequence information for my custom AbSeq antibody?
The sequence barcode belonging to each antibody will be noted in the product information sheet. In addition to being provided with a reference file used in downstream analysis.