BD FACSDuet? Sample Preparation System

The BD FACSDuet™ sample preparation system provides a powerful new level of performance to move the pace of your lab forward. With pre-analytical automation with standardisation, the BD FACSDuet™ drives consistency and workflow efficiency. The BD FACSDuet™ also allows for automated cocktail preparation, greatly enhancing the flexibility and standardization.  Designed to complement and physically integrate with the BD FACSLyric™ flow cytometer, the BD FACSDuet™ provides a complete walkaway sample-to-answer solution. Through its automation protocol, the BD FACSDuet™ enables higher lab productivity and accuracy in results by minimising manual intervention. The complete workflow traceability provided by the BD FACSDuet™ enables the lab to be compliant with ISO-15189 standards.

Specimen (loading) Carrier (loading) Reagent (loading) Reagent (cocktailing) Lysing (loading) System Features

Delivering Flexibility in Specimen and Tube Type

The BD FACSDuet™ answers the growing needs of the laboratories to handle different types and sizes of specimen tubes and supports the need for consolidation and testing centralisation in hospitals. The BD FACSDuet™ delivers the flexibility that is required by supporting twenty-two (22) different types of blood collection tubes from different manufacturers.

The choice includes BD Vacutainer®, Sarstedt S-Monovette®, Greiner and Streck tubes.

Tube Type Tube size

Cat. No. (For dimensional reference only)

BD Vacutainer? 13 x 75 mm

366664 - plastic

366667 - plastic

367671 - glass

BD Hemogard?


BD Hemogard?

13 x 100 mm

367886 - plastic

368660 - plastic

367729 - glass

366450 - glass

BD Hemogard?


BD Hemogard?


16 x 100 mm

366643 - plastic

367880 - plastic

366480 - glass

BD Hemogard?



Greiner 13 x 75 mm



Premium cap

Non-ridged pull cap

13 x 100 mm



Premium cap

Non-ridged pull cap

16 x 100 mm 455084 Non-ridged pull cap



2.7 mL, 11 x 66 mm 05.1167, 05.1167.001 N/A
2.6 mL, 13 x 65 mm 04.1901, 04.1901.001 N/A
3.4, 13 x 65 mm 04.1914, 04.1914.001 N/A
4.9 mL, 13 x 90 mm 04.1931, 04.1931.001 N/A
4.0 mL, 15 x 75 mm 03.1068, 03.1068.001 N/A




5.0 mL, 13 x 75 mm


(5 mL, 6-tube pack)

2 mL Flat


(2 mL, 6-tube pack)



Specimen management is driven by tube adaptors. Seven (7) tube adaptors are available; they are barcoded and color coded for easy identification; they guarantee accurate tube piercing and sample volume dispensing.

Adapter label

Compatible specimen tubes

D13 L65

Sarstedt 2.6 mL and 3.4 mL

D11 L66

Sarstedt 2.7 ml

D13 L75

BD Vacutainer 2.0 mL, 3.0 mL and 4.0 mL & Greiner 2.0 mL and 3.0 mL & Streck 5.0 mL

D15 L75

Sarstedt 4.0 mL

D13 L90

Sarstedt 4.9 mL

D13 L100

BD Vacutainer & Greiner 6.0 mL and 7.0 mL

D16 L100

BD Vacutainer 9.5mL and 10mL & Greiner 9.0 and 10.0 mL

Driving Workflow Efficiency and Throughput

Up to 40 specimen tubes can be accommodated at any given time in 4 racks holding 10 specimens each, driving workflow efficiency and throughput.

Two type of specimen racks are available, of which one is specifically designed for pediatric tubes(B)

  • A. FACSDuet Barcode Scanning_no numbers

  • B.  FACSDuet Barcode Scanning_pediatrics tube

Specimen traceability and automated worklist creation are guaranteed by 5 different barcodes on the specimen rack, identifying:

      1. Rack
      2. Tube position
      3. Empty position
      4. Adaptor
      5. Specimen
FACSDuet Barcode Scanning_with numbers

Automated barcode scanning during rack insertion enables:

    • Patient specimen traceability
    • Pipetting management
    • Retrieving patient and assay information from Laboratory Information System (LIS) for automated worklist creation

Allowing for Throughput and Traceability of the Samples and Worklist

Worklist traceability is enabled by the scanning of the carrier barcode that is associated with a specific worklist and defined specimens.

Secondary tubes are loaded in the BD FACSDuet™ onto 30 and/or 40 tube sample carriers, compatible with the BD FACSLyric™ loader and in the footprint of 96 well plate.

Barcoded secondary tubes are scanned to guarantee sample traceability and association to the specimen and the worklist

Up to 3 carriers can be present at the same time inside the BD FACSDuet, allowing for increased sample throughput.

Refer to Technical Specifications for more information.

Delivering Reagent Management and Flexibility in Panel Design

By accommodating up to 46 reagent vials in 2 separate racks, the BD FACSDuet™ delivers flexibility in antibody panel design and walkaway capabilities.

Vial adaptors allow the use of reagent vials of different sizes.

In addition to the one on the antibody vial (4), the reagent rack carries 3 barcodes: one identifying the rack (1), another the reagent position (2) and a third identifying the empty position (3).

Barcodes on reagent racks and antibody vials drive reagent traceability, efficient antibody management and reduction of reagent waste.

FACSDuet - Features - Reagent- Loader Image 1

Cap racks are available for avoiding the risk of antibody cross-contamination due to cap misplacement

FACSDuet - Features - Reagent- Loader Image 2

The cocktailing functionality allows on-board, automated antibody cocktail preparation, eliminating the risk of errors due to manual pipetting and enabling the automated recording of all used antibodies for easier tracking and auditing.  Part number, lot number and expiry date of the cocktail – as defined by the user – are also tracked and recorded to ensure quality and consistency in laboratory workflow. 

Each antibody cocktail can be made from a maximum of 45 unique conjugates in a single cocktailing vial.  Users’ cocktail recipes are stored in the software and available for subsequent preparations of the same cocktail. Once prepared, the cocktail is considered as any other reagent and available in the user reagent library for selection.

Cocktails designed to contain more than one BD Horizon™ Brilliant dye can include automated addition of BD Horizon™ Brilliant Stain Buffer or BD Horizon™ Brilliant Stain Buffer Plus, ensuring dye stability and staining performance.

The cocktailing software is an integral part of the BD FACSDuet software, and is as intuitive and easy-to-use as the rest of the software.

Cocktailing Home screen

Cocktail Entry screen

Cocktail Library Recipe screen

Load Reagent screen

The lysing solution is contained in a 1 litre tank.

The tank is designed with a built-in funnel for easy filling and avoidance of spillover.

FACSDuet - Features - Lysing - Image

The BD FACSDuet™ delivers

  • a) Data Reproducibility with standardization of processes and protocols
  • b) Consistency in operations with an intuitive software interface minimising operator variations and the need for training. System automation eliminates manual steps and reduces the risk of errors.
  • c) Efficiency and productivity with predictable sample throughput; the continuous specimen loading enables predictable volume processing with creation of lab test volume capacity
  • d) Traceability of specimens, samples, reagent, cocktails and worklists eliminates manual data transcription and risk of errors, enabling conformity to the ISO 15189 standards
  • e) Modularity and flexibility to accommodate any lab need

Sample preparation system

Item Description Cat. No.
BD FACSDuetTM Sample Prep. System Sample preparation system for all Lyse-No-Wash applications. Includes kit for physical integration
with the BD FACSLyricTM flow cytometer. Also includes a starter accessory kit:
Reagent Loading Rack (Set of 2 racks)
Specimen Loading Rack (Set of 4 racks)
13x75 Specimen Tube Adapters (4 packs of 10 ea.)
BD FACSLyric Secondary Tube Rack (30 Tube Rack) (Set of 4 racks)
Handheld Barcode Scanner with Stand
Lysing Solution 1L Tank
10L DI Water Tank
10L Waste Tank
10L Saline Tank
50mL Bleach Container
50mL Chilled Saline Container
Documentation Kit (IFU, User Guide, SPG, SLG)
Panel PC

Optional components

Item Description Cat. No.
Table (recommended when BD FACSDuet and
BD FACSLyric are a physically integrated system)
200.0cm L x 97.0cm D x 86.35cm H
Table includes BD FACSLyric flat screen desk support,
dripping plate with receptacles, 2 chariots for BD FACSDuet
and BD FACSLyric tanks
Secondary Tube Labels Printer Printer generating labels for 12x75mm secondary tubes 662609
Handheld Barcode Scanner, with Stand External barcode reader for both BD FACSLyric and BD FACSDuet 663058
Uninterruptible Power Manager (UPM) 1440VA 230V IN/OUT 663407
Panel PC Replacement touch screen PC 663211


Item Description Cat. No.
Specimen Loading Rack
Specimen Loading Rack (Set of 4) Set of 4 racks for loading primary specimen tubes. Does not include tube adaptors 662592
Streck Cyto-Chex specimen Loading Rack (Set of 4) Set of 4 racks for loading Streck Cyto-Chex tubes. 662593
Specimen Rack Adapters
13x65 Specimen Tube Adapters (Set of 10) 13mmx65mm adapter for Sarstedt 2.6mL and 3.4mL tubes 662594
11x66 Specimen Tube Adapters (Set of 10) 11mmx66mm adapter for Sarstedt 2.7mL tubes 662595
13x75 Specimen Tube Adapters (Set of 10) 13mmx75mm adapter for BD 2mL, 3mL and 4mL, Greiner 2mL and 3mL, Streck 5mL tubes 662596
15x75 Specimen Tube Adapters (Set of 10) 15mmx75mm adapter for Sarstedt 4mL tubes 662597
13x90 Specimen Tube Adapters (Set of 10) 13mmx90mm adapter for Sarstedt 4.9mL tubes 662598
13x100 Specimen Tube Adapters (Set of 10) 13mmx100mm adapter for BD and Greiner 6mL and 7mL tubes 662600
16x100 Specimen Tube Adapters (Set of 10) 16mmx100mm adapter for BD 9.5mL and 10mL and Greiner 9mL and 10mL tubes 662599
Secondary Tube Racks
BD FACSLyric Secondary Tube Rack (30 Tube Rack) Secondary tube rack to accommodate 30 12x75mm tubes 651314
BD FACSLyric Secondary Tube Rack (40 Tube Rack) Secondary tube rack to accommodate 40 12x75mm tubes 651319
Reagent Racks
Reagent Loading Rack (Set of 2) Set of 2 racks, each accommodates 23 reagent vials. Adapters not included 662664
Reagent Rack Adapters
Reagent Rack Adapter Type 1 (Pack of 10) Adapter to accommodate BD TruCountTM controls, and 4mL BD HorizonTM Brilliant Stain Buffer 663260
Reagent Rack Adapter Type 2 (Pack of 10) Adapter to accommodate 0.5mL and 2mL Pharmingen Reagent Vials 663261
Miscellaneous Accessories
Lysing Solution 1L Tank 1L tank for lysing solution 662602
10L DI Water Tank 10L tank for DI water 662603
10L Waste Tank 10L tank for waste 662604
10L Saline Tank 10L tank for saline 662605
50mL Bleach Container 50mL bleach container for end of day clean cycle 662606
50mL Saline Container 50mL container for saline (in the reagent bay) 662607

Maintenance kits

Item Description Cat. No.
Peristaltic Pump Tubing Replacement Kit Kit for replacement of the tubing for peristaltic pumps 662613
Specimen Probe Replacement Kit Kit for replacement of specimen pipettor probe 662614
Syringe Pump Valve Replacement Kit Kit for replacement of the syringe valve pump 663247
Syringe Pump Replacement Kit Kit for replacement of the 500μL syringe 663249
DI Water Filter Replacement Kit Kit for replacement of the DI water filter 663923
Maintenance kit Kit for 6 month maintenance (includes 662614, 662613, 663247, 663249 and 663923) 663926

System and LIS connectivity

Item Description Cat. No.
BD FACSLinkTM Basic Software + 1 Year Maintenance (EU only) BD FACSLink basic software + 2 connections + 1 year maintenance 663061
BD FACSLink Computer Assembly (EU only) Computer assembly for BD FACSLink software 663874
BD FACSLink User License + 1 Year Maintenance (EU only) Extra connection license (optional, for multiple instruments) 663062
BD FACSLink Computer Assembly (non-EU) Computer assembly for BD FACSLink software 663875
BD FACSLink Software Package (non-EU) BD FACSLink software for BD FACSLyric. Allows for connection to LIS 663893
BD FACSLink Connection License for Additional Instruments (non-EU) Extra BD FACSLink connection license (optional, for multiple instruments) 663625
BD FACSLink Software Media Kit (for all regions except EU, US, Canada) Media kit required for first time installations or software upgrades of BD FACSLink 663727

BD FACSDuet? System Testimonials

See what customers are saying about the BD FACSDuet™.

FACSDuet - Accreditation The BD FACSDuet will allow us to be compliant with UKAS, the accreditation service, and ISO 15189. Dr. Tim Farren, Head of Immunophenotyping, Barts Heath NHS Trust.
Watch Now→
FACSDuet - Teaser “The automation allows faster process, faster throughput, increased capacity”, adds Nicole Gresham, Associate Director of Pathology at the Barts Health NHS Trust, UK.
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FACSDuet - Automation BD FACSDuet™ - “A real game changer in flow cytometry” , says Chris Scott – Barts Health NHS Trust, UK
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The integrated BD FACSDuet-BD FACSLyric system The integrated BD FACSDuet-BD FACSLyric system satisfies our requirements of increasing efficiency, standardizing processes and improving quality. Mrs. Debbie Bruno – CD4 Laboratory Manager – Ampath Laboratories
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The integrated BD FACSDuet-BD FACSLyric system PART 2-Efficiency, Quality, Automation and Traceability are key to reduce Laboratory Turnaround Time. Mrs. Debbie Bruno – CD4 Laboratory Manager – Ampath Laboratories
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FACSDuet - Testimonials - Dr. Bloxham “The BD FACSDuet enables us to increase our laboratory’s efficiency and throughput, replacing manual processes with automated ones that are traceable and that can be automatically transferred and recorded in our laboratory information system providing a complete audit trail"

—Mr. D. Bloxham – Principle Biomedical Scientist – HODS Cambridge University Hospital NHS Trust – Cambridge - UK
FACSDuet - Testimonials - Farren “At last, a fully integrated end-to-end walkaway solution, which is going to make our lives significantly easier. It reduces errors in both the pre-analytical and analytical phases, leading to increased productivity and efficiency, but most importantly provides a comprehensive fully audit-able account of the whole process. Finally a solution that creates capacity to allow us to meet our key performance indicators”

—Dr. T. Farren - Head of Immunophenotyping (SIHMDS) Barts Health NHS Trust, UK
FACSDuet - Testimonials - Scott “I require all the monoclonal antibodies used, the ancillary reagents, tubes, the QC materials to be linked to an exact patient…this is an essential laboratory process and it is also mandatory in order to maintain ISO 15189 accreditation"

—Mr. C. Scott – Lead Biomedical Scientist, Immunology Dep. Barts Health NHS Trust, UK
FACSDuet - Testimonials - Bloxham “What is the potential impact of the BD FACSDuet? It removes our manual processes, increases our efficiency and throughput, and gives us control in standardization of processes. It’s a controlled environment ”

—Mr. D. Bloxham – Principle Biomedical Scientist – HODS Cambridge University Hospital NHS Trust – Cambridge - UK

Unless otherwise noted the products are for in Vitro Diagnostic Use. CE marked in compliance with the In Vitro Diagnostic Medical Device Directive 97/79/EC

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